There are both direct and indirect ways to measure the presence of EOS® in the aquifer.  Apparent turbidity, observed as a faint white tint in performance monitoring wells, can sometimes be used as a visual indicator of EOS® presence.  Laboratory analysis for total organic carbon (TOC) and volatile fatty acids (VFA) can also provide direct indications of the presence of substrate.  Indirectly, changes in groundwater geochemisty as a result of formation of anaerobic reducing conditions suggest that EOS® is present.